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Proceedings Paper

Heme-tryptophan relationships in hemoglobin explored by frequency-domain time-resolved fluorescence at 10-GHz resolution
Author(s): Enrico Bucci; Zygmunt Gryczynski; Enrico Gratton; Todd Tenenholz
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Paper Abstract

The time-resolved fluorescence spectroscopy of human and bovine oxy- and deoxyhemoglobins was measured in either 0.03 M phosphate buffer or 0.03 M borate buffer between pH 6.5 and 9.2. A frequency resolved fluorometer was used with bandwidth up to 10 GHz. Excitation was at 294 nm, the emission was monitored through a broad band interference filter centered at 335 nm, coupled to a cut-off filter at 316 nm. In all cases, the best simulations were obtained with two discrete exponential decays, one near 30 ps, and the other of several hundred ps. In human hemoglobin, the longer component showed a substantial lengthening upon removal of oxygen. In bovine hemoglobin, the shorter component decreased upon deoxygenation. It was possible to infer that the shorter lifetimes originated from the average intrachain distances. However, the hemes at this longer distance would become the main acceptors of energy transfer only when the energy transfer at intrasubunit distance is inhibited. It is suggested that this is due to the presence of `disordered' heme in the system.

Paper Details

Date Published: 1 April 1992
PDF: 8 pages
Proc. SPIE 1640, Time-Resolved Laser Spectroscopy in Biochemistry III, (1 April 1992); doi: 10.1117/12.58274
Show Author Affiliations
Enrico Bucci, Univ. of Maryland School of Medicine (United States)
Zygmunt Gryczynski, Univ. of Maryland School of Medicine (United States)
Enrico Gratton, Univ. of Illinois/Urbana-Champaign (United States)
Todd Tenenholz, Univ. of Maryland School of Medicine (United States)

Published in SPIE Proceedings Vol. 1640:
Time-Resolved Laser Spectroscopy in Biochemistry III
Joseph R. Lakowicz, Editor(s)

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