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Proceedings Paper

Photoinduced electron transfer and fluorescence mechanisms in covalently linked polynuclear aromatic-nucleotide complexes
Author(s): Nicholas E. Geacintov; Bing Mao; Luisa L. France; Rushen Zhao; Junxin Chen; Tong Ming Liu; Nai-Qi Ya; Leonid A. Margulis; John Clark Sutherland
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Paper Abstract

The fluorescence of polycyclic aromatic hydrocarbon-nucleic acid complexes is quenched by photoinduced electron transfer mechanisms in aqueous solutions at ambient temperatures. These effects are illustrated with the biologically important compound benzo[a]pyrene-7,8-diol-9,10-epoxide (BPDE), a mutagenic and carcinogenic metabolite of the environmental pollutant benzo[a]pyrene, which forms covalent mutagenic lesions with 2'-deoxyguanosine (dG) residues in DNA. The dependence of the fluorescence yield and fluorescence decay times of the covalent model adduct (+)-trans- BPDE-N2-dG as a function of temperature and methanol/water composition are described. Because of the sensitivity of the fluorescence of the pyrenyl residue to the polarity of the microenvironment, the magnitude of the fluorescence yield can be used to distinguish between highly hydrophobic (e.g., intercalation) and other more solvent-exposed BPDE- nucleic acid binding sites.

Paper Details

Date Published: 1 April 1992
PDF: 10 pages
Proc. SPIE 1640, Time-Resolved Laser Spectroscopy in Biochemistry III, (1 April 1992); doi: 10.1117/12.58273
Show Author Affiliations
Nicholas E. Geacintov, New York Univ. (United States)
Bing Mao, New York Univ. (United States)
Luisa L. France, Brookhaven National Lab. (United States)
Rushen Zhao, New York Univ. (United States)
Junxin Chen, New York Univ. (United States)
Tong Ming Liu, New York Univ. (United States)
Nai-Qi Ya, New York Univ. (United States)
Leonid A. Margulis, New York Univ. (United States)
John Clark Sutherland, Brookhaven National Lab. (United States)

Published in SPIE Proceedings Vol. 1640:
Time-Resolved Laser Spectroscopy in Biochemistry III
Joseph R. Lakowicz, Editor(s)

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