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Proceedings Paper

Fluorescence decay and depolarization of probes in membranes
Author(s): Dmitri D. Toptygin; Jaroslava Svobodova; Ivo Konopasek; Ludwig Brand
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Paper Abstract

Radiative decay rate depends on the efficiency of coupling between the emission dipole and the electromagnetic field. Therefore, in optically discontinuous and/or anisotropic environments the radiative rate is dependent on the orientation of the emission dipole. Single-bilayer phospholipid membranes are interesting systems for the study of this phenomenon because in these systems the functional dependence of the radiative rate is simple and the effect is stronger than in liquid crystals. The orientational dependence of the radiative rate results in a non-exponential total fluorescence intensity decay. Orientational distribution of the probe in the gel-phase membrane can be determined from the total intensity decay. In the liquid-phase membrane the coupling between decay and rotation leads to a polarized intensity decay behavior entirely different than that in isotropic media. Experimental data are consistent with every theoretical prediction.

Paper Details

Date Published: 1 April 1992
PDF: 13 pages
Proc. SPIE 1640, Time-Resolved Laser Spectroscopy in Biochemistry III, (1 April 1992); doi: 10.1117/12.58270
Show Author Affiliations
Dmitri D. Toptygin, Johns Hopkins Univ. (United States)
Jaroslava Svobodova, Johns Hopkins Univ. (United States)
Ivo Konopasek, Johns Hopkins Univ. (United States)
Ludwig Brand, Johns Hopkins Univ. (United States)

Published in SPIE Proceedings Vol. 1640:
Time-Resolved Laser Spectroscopy in Biochemistry III
Joseph R. Lakowicz, Editor(s)

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