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Proceedings Paper

Study of the interaction of 5,7,7,12,14,14-hexamethyl-1,4,8,11-tetraaza-cyclotetradeca-4,11-dienatocopper with DNA by ethidium fluorescence spectroscopy
Author(s): Changlin Liu; Juan Zou; Hui-Bie F. Xu
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Paper Abstract

The interaction of copper(II) and its 5,7,7,12,14,14-hexamethyl-1,4,8,11-tetraazacyclo- tetradeca-4,11-diene(HTDE) complex with calf thymus DNA has been investigated by ethidium fluorescence spectroscopy. The experimental data shows that Cu(II), Cu(II)HTDE, and Cu(II)HTDE-thiol-hydrogen peroxide cause ethidium to dissociate from its host molecule and decrease the relative emission intensity of DNA-ethidium. Two different interaction modes could be deduced from the Cu(II) characteristics, the structure, steric constraint of Cu(II)HTDE, and the composition of the system. The evidence accumulated suggests that Cu(II)HTDE intercalates predominantly into the double-helical DNA in the absence of a reducing agent and hydrogen peroxide, and that it binds within the minor groove of DNA in the presence of RSH and H2O2.

Paper Details

Date Published: 1 April 1992
PDF: 8 pages
Proc. SPIE 1640, Time-Resolved Laser Spectroscopy in Biochemistry III, (1 April 1992); doi: 10.1117/12.58256
Show Author Affiliations
Changlin Liu, Huazhong Univ. of Science and Technology (China)
Juan Zou, Huazhong Univ. of Science and Technology (China)
Hui-Bie F. Xu, Huazhong Univ. of Science and Technology (China)

Published in SPIE Proceedings Vol. 1640:
Time-Resolved Laser Spectroscopy in Biochemistry III
Joseph R. Lakowicz, Editor(s)

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