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Proceedings Paper

Dilemma of correlating fluorescence quantum yields and intensity decay times in single-tryptophan mutant proteins
Author(s): Arthur G. Szabo; C. Faerman
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Paper Abstract

The relationship between the fluorescence quantum yield, (phi) f, radiative lifetime, (tau) r, and excited singlet state lifetime, (tau) s, is considered to be fundamental in fluorescence studies. Because of advances in instrumentation and data analysis the intensity decay times of tryptophan in proteins can be determined with a high degree of confidence and accuracy. Site directed mutagenesis allows one to place tryptophan residues in different locations within proteins. In several mutant single tryptophan containing proteins studied in our laboratory it has often been noted that there is a lack of a proportional correlation between the measured fluorescence quantum yield and the mean fluorescence decay time. This data and examples from the literature are presented and rationalizations are discussed. The intent is to stimulate experimental design and theoretical studies. This will lead to new intuitions from fluorescence studies of mutant proteins regarding the interrelationship of their structure, dynamics, and function.

Paper Details

Date Published: 1 April 1992
PDF: 11 pages
Proc. SPIE 1640, Time-Resolved Laser Spectroscopy in Biochemistry III, (1 April 1992); doi: 10.1117/12.58202
Show Author Affiliations
Arthur G. Szabo, National Research Council Canada (Canada)
C. Faerman, National Research Council Canada (Canada)

Published in SPIE Proceedings Vol. 1640:
Time-Resolved Laser Spectroscopy in Biochemistry III
Joseph R. Lakowicz, Editor(s)

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