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Proceedings Paper

Biological laser printing as an alternative to traditional protein arrayers
Author(s): Jason A. Barron; Henry D. Young; Brad R. Ringeisen; Dana D. Dlott; Dave B. Krizman
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Paper Abstract

Current proteomics experiments often rely upon printing techniques such as ink jet, pin, or quill arrayers that were developed for the creation of cDNA microarrays. These techniques often do not meet the spotting requirements needed for successful high throughput protein identification and profiling. The Naval Research Laboratory has developed an alternative to these commercially available arrayers that does not rely upon a solid pin or capillary-based fluidics. This presentation describes experiments demonstrating that biological laser printing, or BioLP, is capable of depositing microarrays of proteins rapidly and efficiently. This technique utilizes a focused laser pulse to obtain micron-scale resolution rather than a pin or orifice, thereby eliminating clogging and protein loss commonly encountered in commercially available printers. The speed and spot-to-spot reproducibility of the printer is comparable to other techniques, while the minimum spot diameter and volume per printed droplet is significantly less at 30 microns and ~500 fL, respectively. The transfer of fluid by BioLP occurs through a fluid jetting mechanism, as observed by high-speed images of the printing process. In addition, printed biotinylated bovine serum albumin is identified through immunoassay and observed by fluorescent detection. These results indicate that BioLP holds promise as a novel protein printer for use in a wide range of applications in the proteomics field.

Paper Details

Date Published: 29 March 2005
PDF: 9 pages
Proc. SPIE 5699, Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III, (29 March 2005); doi: 10.1117/12.580101
Show Author Affiliations
Jason A. Barron, Naval Research Lab. (United States)
Henry D. Young, Naval Research Lab. (United States)
Brad R. Ringeisen, Naval Research Lab. (United States)
Dana D. Dlott, Univ. of Illinois at Urbana-Champaign (United States)
Dave B. Krizman, Expression Pathology, Inc. (United States)

Published in SPIE Proceedings Vol. 5699:
Imaging, Manipulation, and Analysis of Biomolecules and Cells: Fundamentals and Applications III
Dan V. Nicolau; Dan V. Nicolau; Jörg Enderlein; Ramesh Raghavachari; Robert C. Leif; Daniel L. Farkas, Editor(s)

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