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Proceedings Paper

Optical classification of bioaerosols using UV fluorescence and IR absorption spectroscopy
Author(s): Alan L. Huston; Vasanthi Sivaprakasam; Cathy S. Scotto; Horn-Bond Lin; Jay D. Eversole; Abraham Schultz; Jeff M. Willey
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Paper Abstract

A partnership that includes the Naval Research Laboratory (NRL), MIT Lincoln Laboratories and the Edgewood Chemical and Biological Command is engaged in an effort to develop optical techniques for the rapid detection and classification of biological aerosols. This paper will describe two efforts at NRL: development of an improved UV fluorescence front-end trigger and the use of infrared absorption spectroscopy to classify biological aerosol particles. UV Laser-induced fluorescence (UVLIF) has been demonstrated to provide very high sensitivity for differentiating between biological and inorganic aerosol particles. Unfortunately, current UVLIF systems have unacceptably high false alarm rates due to interferences from man made and naturally occurring organic and biological particulates. We have developed a two-wavelength, UVLIF technique that offers a higher level of discrimination than is possible using single wavelength UVLIF. Infrared absorption spectroscopy coupled with multivariate analysis demonstrates a high potential for differentiation among members of biological and chemical sample classes. Two-wavelength UVLIF in combination with the IR interrogation of collected bioaerosols could provide a rapid, reagentless approach to specific classification of biological particles according to an operational level of discrimination - the degree of particle characterization required in order to signal the presence of pathogenic material.

Paper Details

Date Published: 29 December 2004
PDF: 12 pages
Proc. SPIE 5617, Optically Based Biological and Chemical Sensing for Defence, (29 December 2004); doi: 10.1117/12.578062
Show Author Affiliations
Alan L. Huston, Naval Research Lab. (United States)
Vasanthi Sivaprakasam, Naval Research Lab. (United States)
Cathy S. Scotto, Naval Research Lab. (United States)
Horn-Bond Lin, Naval Research Lab. (United States)
Jay D. Eversole, Naval Research Lab. (United States)
Abraham Schultz, Naval Research Lab. (United States)
Jeff M. Willey, Naval Research Lab. (United States)

Published in SPIE Proceedings Vol. 5617:
Optically Based Biological and Chemical Sensing for Defence
John C. Carrano; Arturas Zukauskas, Editor(s)

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