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Proceedings Paper

Disposable laser-diffraction ektacytometry to measure red blood cell deformability
Author(s): Sehyun Shin; Yunhee Ku; Myungsu Park; Lijuan Zhang; Joohee Jang; Jangsoo Suh
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Paper Abstract

The laser-diffraction technique has been applied to design a slit rheometer for measuring red blood cell deformability over a range of shear stress. A laser beam traverses a diluted blood suspension and is diffracted by RBCs in the volume. The diffraction patterns are captured by a CCD-video camera, linked to a frame grabber integrated with a computer. When deforming under decreasing shear stress, RBCs change gradually from the prolate ellipsoid towards a circular biconcave morphology. Both the laser-diffraction image and pressure were measured with respect to time. Which enable to determine the elongation index (EI) and the shear stress. The range of shear stress is 0~35 Pa and the measuring time is less than 2 min. The elongation index (EI) is determined from an isointensity curve in the diffraction pattern using an ellipse-fitting program. The key advantage of this design is the incorporation of a disposable element that holds the blood sample, which enables the present system to be easily used in a clinical setting.

Paper Details

Date Published: 18 January 2005
PDF: 9 pages
Proc. SPIE 5630, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II, (18 January 2005); doi: 10.1117/12.575957
Show Author Affiliations
Sehyun Shin, Kyungpook National Univ. (South Korea)
Yunhee Ku, Kyungpook National Univ. (South Korea)
Myungsu Park, Kyungpook National Univ. (South Korea)
Lijuan Zhang, Kyungpook National Univ. (South Korea)
Joohee Jang, Kyungpook National Univ. (South Korea)
Jangsoo Suh, Kyungpook National Univ. (South Korea)

Published in SPIE Proceedings Vol. 5630:
Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II
Britton Chance; Mingzhe Chen; Arthur E. T. Chiou; Qingming Luo, Editor(s)

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