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Proceedings Paper

Experimental measurement of the trapping force acting on a yeast cell with a lensed optical fiber probe
Author(s): Zhaohui Hu; Jia Wang; Jinwen Liang
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Paper Abstract

In conventional optical tweezers system a high numerical aperture (NA) objective is employed both to image and to generate a gradient force toward the focus on sample particles, so the system is complex and expensive especially for the multi-optical-tweezers system. We built a novel simple optical trapping system based on a lensed optical fiber probe. This new method offers several other advantages over the conventional optical tweezers. The trapping system we built includes a laser coupling unit, a multi-dimensional probe manipulating unit, a sample nano-positioning unit, and a microscopy imaging unit. Based on the system, a yeast cell is trapped and manipulated on the chamber bottom by the lensed fiber probe, and the optical trapping forces acting on the yeast cell as a function of the offset are measured and discussed in different directions by the static method and the dynamic one respectively with various powers. The results by the two measurement methods coincide with each other, and the detail experimental procedure and the data processing of the two methods are introduced in this paper.

Paper Details

Date Published: 18 January 2005
PDF: 8 pages
Proc. SPIE 5630, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II, (18 January 2005); doi: 10.1117/12.575668
Show Author Affiliations
Zhaohui Hu, Tsinghua Univ. (China)
Jia Wang, Tsinghua Univ. (China)
Jinwen Liang, Tsinghua Univ. (China)

Published in SPIE Proceedings Vol. 5630:
Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II
Britton Chance; Mingzhe Chen; Arthur E. T. Chiou; Qingming Luo, Editor(s)

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