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Proceedings Paper

Distribution of fluorescent probe molecules throughout the phospholipid membrane depth
Author(s): Nicolai A. Nemkovich; Anatoly Nikolaevich Rubinov; M. G. Savvidi; V. I. Tomin; Nina V. Shcherbatska
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Paper Abstract

A method to determine the distribution function shape of the luminescent probe molecules over biological membrane depth is suggested. It is based on: - the unhomogeneous model of the probe ensemble which permits its selection alteration selectively of the position and distribution of excited probe molecules by the wavelength of the excitation light - the use of the nonradiative electronic energy transfer (NEET) mechanism from probe molecules in vesicules to the acceptor ones in a buffer solution. The distribution function of the donor-excited molecules for different wavelength of the excitation light was calculated. Using the spectra of probe molecules at different Ae and Lippert''s equation the values of the dielectric constant of the membrane e were obtained for different regions. 1.

Paper Details

Date Published: 1 May 1991
PDF: 4 pages
Proc. SPIE 1403, Laser Applications in Life Sciences, (1 May 1991); doi: 10.1117/12.57387
Show Author Affiliations
Nicolai A. Nemkovich, B. I. Stepanov Institute of Physics (Belarus)
Anatoly Nikolaevich Rubinov, B. I. Stepanov Institute of Physics (Belarus)
M. G. Savvidi, B. I. Stepanov Institute of Physics (Belarus)
V. I. Tomin, B. I. Stepanov Institute of Physics (Belarus)
Nina V. Shcherbatska, B. I. Stepanov Institute of Physics (Belarus)

Published in SPIE Proceedings Vol. 1403:
Laser Applications in Life Sciences
Sergei A. Akhmanov; Marina Yu. Poroshina, Editor(s)

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