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Proceedings Paper

Novel confocal microarray scanner for fluorescence detection
Author(s): Liqiang Wang; Xuxiang Ni; Zukang Lu
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Paper Abstract

A novel confocal microarray scanner was introduced, which employed a 532nm laser and a 635nm laser to excite Cy3 and Cy5 fluorophores respectively. The fluorescent signal was detected using a photomultiplier tube (PMT) sequentially. One dimension scanning of the microarray slide was performed by a telecentric objective with a moving coil optical scanner; the other dimension was scanned by a stepping motor driving the precise guidance. Experimental results show that scanning resolution of the presented microarray scanner can reach 5 microns, its dynamic range is near 4 orders of magnitude and the limit of detection is 1.12 about fluors per square micron. The cross-talk error is eliminated almost completely by its sequential scanning mode.

Paper Details

Date Published: 18 January 2005
PDF: 7 pages
Proc. SPIE 5630, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II, (18 January 2005); doi: 10.1117/12.573428
Show Author Affiliations
Liqiang Wang, Zhejiang Univ. (China)
Xuxiang Ni, Zhejiang Univ. (China)
Zukang Lu, Zhejiang Univ. (China)

Published in SPIE Proceedings Vol. 5630:
Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II
Britton Chance; Mingzhe Chen; Arthur E. T. Chiou; Qingming Luo, Editor(s)

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