Share Email Print

Proceedings Paper

Spectroscopy of proteins on surfaces: implications for protein orientation and protein/protein interactions
Author(s): Therese M. Cotton; Bernard Rospendowski; Vicki Schlegel; Robert A. Uphaus; Danli L. Wang; Lars Eng; Marion T. Stankovich
Format Member Price Non-Member Price
PDF $17.00 $21.00

Paper Abstract

Several approaches for stabilizing three hemeproteins (cytochrome c myoglobin and cytochrome c3) on a silver surface were developed. Methodology for preventing denaturation of complex biomolecules such as surface modification by small molecules is often required in order to obtain surface-enhanced resonance Raman scattering (SERRS) spectra that are relevant to the functional molecule. The optimal approach depends upon the stability of the protein. For example cytochrome is native on a number of different substrates (silver electrodes sols and thin-films) whereas myoglobin is most stable on silver sols. Because SERRS detects changes in the interactions of the heme group with the protein the greater stability of cytochrome as compared to myoglobin probably reflects the fact that the heme is covalently bound in the former. Surface selection rules were used to analyze the orientation of the heme group of several proteins relative to the silver surface. A comparison of the changes in the relative intensities of bands in the SERRS spectra of cytochrome indicates that the protein orientation is sensitive to the presence of lipids or other proteins (avidin) on the silver surface. 1.

Paper Details

Date Published: 1 May 1991
PDF: 9 pages
Proc. SPIE 1403, Laser Applications in Life Sciences, (1 May 1991); doi: 10.1117/12.57297
Show Author Affiliations
Therese M. Cotton, Iowa State Univ. (United States)
Bernard Rospendowski, Iowa State Univ. (United States)
Vicki Schlegel, Iowa State Univ. (United States)
Robert A. Uphaus, Iowa State Univ. (United States)
Danli L. Wang, Univ. of Minnesota (United States)
Lars Eng, Royal Institute of Technology (Sweden)
Marion T. Stankovich, Univ. of Minnesota (United States)

Published in SPIE Proceedings Vol. 1403:
Laser Applications in Life Sciences
Sergei A. Akhmanov; Marina Yu. Poroshina, Editor(s)

© SPIE. Terms of Use
Back to Top
Sign in to read the full article
Create a free SPIE account to get access to
premium articles and original research
Forgot your username?