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Proceedings Paper

Electrophoretic mobility and conformational changes of DNA in agarose gels
Author(s): Benjamin Chu; Zhulun Wang
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Paper Abstract

We present observations of time-dependent electrophoretic mobility and conformational changes of a polyelectrolyte DNA in agarose gels under an applied electric field. The short-time averaged electrophoretic mobility can be measured by means of movement of fluorescence pattern after photobleaching (MOFPAP) while the average conformational change of an assembly of polyelectrolyte chains can be revealed by means of transient electric birefringence (TEB) which measures the rise and fall of the time-dependent optical anisotropy of the polyelectrolyte chains under an applied electric field. The results from a combination of the two techniques (MOFPAP and TEB) are discussed in terms of its implications on the mechanisms responsible for pulsed-field gel electrophoresis. 1.

Paper Details

Date Published: 1 May 1991
PDF: 10 pages
Proc. SPIE 1403, Laser Applications in Life Sciences, (1 May 1991); doi: 10.1117/12.57294
Show Author Affiliations
Benjamin Chu, SUNY/Stony Brook (United States)
Zhulun Wang, SUNY/Stony Brook (United States)

Published in SPIE Proceedings Vol. 1403:
Laser Applications in Life Sciences
Sergei A. Akhmanov; Marina Yu. Poroshina, Editor(s)

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