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Proceedings Paper

Use of OCT to study tumor cell growth in 3D membranes
Author(s): Ying Yang; Josep Sule-Suso; Alicia J. El Haj; Paul R. Hoban; Ruikang K. Wang
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Paper Abstract

Mesothelioma is a tumour, usually fatal, which arises from and invades the pleural membrane. Investigation of the tumour cell-membrane interaction will greatly increase our understanding of the invasion mechanisms, which have the potential to improve the management of the disease. In this study, a new imaging technique, optical coherence tomography (OCT), was used to monitor tumour cell invasion in artificial membranes composed of either collagen type I or Matrigel. In parallel, standard histological section analysis was performed to validate the accuracy of the monitoring by OCT. Cross-sectional images from OCT revealed that lung tumour cells invaded only when low cell seeding density (5 x 105) and low collagen concentration (1.5 mg/ml) were combined. The cells could be easily differentiated from the artificial membranes and appeared as either a brighter layer on the top of the membrane or brighter spots embedded within the darker membrane. These cell-membrane morphologies matched remarkably to the standard histological section images. Our results suggest that OCT has a great potential to become a useful tool for fast and robust assessment of cell invasion.

Paper Details

Date Published: 29 July 2004
PDF: 7 pages
Proc. SPIE 5486, ALT'03 International Conference on Advanced Laser Technologies: Biomedical Optics, (29 July 2004); doi: 10.1117/12.571998
Show Author Affiliations
Ying Yang, Keele Univ. School of Medicine (United Kingdom)
Josep Sule-Suso, Keele Univ. School of Medicine (United Kingdom)
Alicia J. El Haj, Keele Univ. School of Medicine (United Kingdom)
Paul R. Hoban, Keele Univ. School of Medicine (United Kingdom)
Ruikang K. Wang, Cranfield Univ. (United Kingdom)

Published in SPIE Proceedings Vol. 5486:
ALT'03 International Conference on Advanced Laser Technologies: Biomedical Optics
Ruikang K. Wang; Jeremy C. Hebden; Alexander V. Priezzhev; Valery V. Tuchin, Editor(s)

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