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Proceedings Paper

Proposal of spectroscopy-tomography of single cell
Author(s): Toshiki Yasokawa; Ichirou Ishimaru; Fumikazu Oohira; Ryoji Hyodo; Hiroaki Kobayashi; Akihiro Hayashi; Y. Inoue; Katsumi Ishizaki
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Paper Abstract

Currently, diagnosis of cancer is performed by biopsy, whereby medical doctors observe a removed specimen, focusing their attention on morphological changes in the cell sequence and cell nuclei. For early cancer, the only effect is a slight increase in the size of the cell nuclei in comparison with normal cells. Based on medical knowledge, it is presumed that an extremely small amount of a specific protein may be contained in a cell nucleus. We propose spectroscopy-tomography of single cell to measure slight changes in this protein. This technology is composed of two elemental technologies, high spatial resolution spectrometry and a precise single cell rotating method. We propose variable phase-contrast spectrometry as the high spatial resolution spectrometry and proximal two-beam optical tweezers as the precise rotating method. By these methods, we can obtain a 3-dimensional distribution of the cell components to a high spatial resolution. We verified the accuracy of variable phase-contrast spectrometry by measuring the height of a diffraction grating. We confirmed that a microsphere can be rotated by proximal two-beam optical tweezers.

Paper Details

Date Published: 25 October 2004
PDF: 10 pages
Proc. SPIE 5604, Optomechatronic Micro/Nano Components, Devices, and Systems, (25 October 2004);
Show Author Affiliations
Toshiki Yasokawa, Kagawa Univ. (Japan)
Ichirou Ishimaru, Kagawa Univ. (Japan)
Fumikazu Oohira, Kagawa Univ. (Japan)
Ryoji Hyodo, Kagawa Univ. (Japan)
Hiroaki Kobayashi, Kagawa Univ. (Japan)
Akihiro Hayashi, Kagawa Univ. (Japan)
Y. Inoue, Kagawa Univ. (Japan)
Katsumi Ishizaki, Kagawa Univ. (Japan)

Published in SPIE Proceedings Vol. 5604:
Optomechatronic Micro/Nano Components, Devices, and Systems
Yoshitada Katagiri, Editor(s)

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