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Proceedings Paper

Supramolecular interfacial architectures for biosensing
Author(s): Fang Yu; Danfeng Yao; Danica Christensen; Thomas Neumann; Eva-Kathrin Sinner; Wolfgang Knoll
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Paper Abstract

This contribution summarizes some of our efforts in designing, assembling and functionally characterizing supramolecular interfacial architectures for bio-affinity studies and for biosensor development. All the surface interaction studies will be based on the recently introduced novel sensor platforms involving surface plasmon fluorescence spectroscopy (SPFS) and -microscopy (SPFM). Emphasis will be put on documenting the distance-dependence of fluorescence intensity at the metal-dielectric interface and utilizing this principle to optimize the conformation/orientation of the interfacial supra-molecular sensor coatings. This is exemplified by a number of examples, including a layer-by-layer assembly system, antibody-antigen interactions, oligonucleotide-oligonucleotide, and oligonucleotide-PCR amplicon hybridization. For practical sensing purposes, a three-dimensionally extended surface coating is then employed to overcome the fluorescence quenching problem on a planar matrix. A commercial dextran layer is shown to be an optimized matrix for SPFS, with an example of a protein-binding study.

Paper Details

Date Published: 29 December 2004
PDF: 14 pages
Proc. SPIE 5593, Nanosensing: Materials and Devices, (29 December 2004); doi: 10.1117/12.570695
Show Author Affiliations
Fang Yu, Max Planck Institute for Polymer Research (Germany)
Danfeng Yao, Max Planck Institute for Polymer Research (Germany)
Danica Christensen, Max Planck Institute for Polymer Research (Germany)
Thomas Neumann, Graffinity Pharmaceuticals AG (Germany)
Eva-Kathrin Sinner, Max Planck Institute of Biochemistry (Germany)
Wolfgang Knoll, Max Planck Institute for Polymer Research (Germany)


Published in SPIE Proceedings Vol. 5593:
Nanosensing: Materials and Devices
M. Saif Islam; Achyut K. Dutta, Editor(s)

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