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Proceedings Paper

The photoinduced conformational change in macromolecules
Author(s): Y. Barabash; V. Kharkyanen; A. Kudrjavtsev; M. A. Zabolotny; Nickolai I. Sokolov
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Paper Abstract

This paper concerns with our measurements of reaction center (RC) volume changes in the course of photoactivation using method of holographic interferometry. As a principal value, we studed change of a solution refraction index (▵n) influenced by the sample volume expansion due to temperature changes (▵nT), light absorption (▵nabs) and changes in the volume of photoactive molecules (▵nV). Using the scheme of temperature compensation we could reduce the task of the case ▵nT<<▵nabs, ▵nV Our estimation for the case of a protein solution yields in relative changes in the molecular volume within 10-3 ÷ 10-2.

Paper Details

Date Published: 20 July 2004
PDF: 7 pages
Proc. SPIE 5507, XVI International Conference on Spectroscopy of Molecules and Crystals, (20 July 2004); doi: 10.1117/12.569840
Show Author Affiliations
Y. Barabash, Institute of Physics (Ukraine)
V. Kharkyanen, Institute of Physics (Ukraine)
A. Kudrjavtsev, Institute of Semiconductor Physics (Ukraine)
M. A. Zabolotny, Taras Shevchenko National Univ. of Kyiv (Ukraine)
Nickolai I. Sokolov, Univ. of Kiev-Mohyla Academy (Ukraine)

Published in SPIE Proceedings Vol. 5507:
XVI International Conference on Spectroscopy of Molecules and Crystals
Galyna O. Puchkovska; Tatiana A. Gavrilko; Olexandr I. Lizengevich, Editor(s)

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