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Proceedings Paper

MACROscopic imaging of tumor xenografts using fluorescence, phase contrast, and transmitted light
Author(s): Paul Constantinou; Trudey Nicklee; David W. Hedley; Brian C. Wilson; Savvas Damaskinos
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Paper Abstract

Recent advances in imaging technology have contributed greatly to biological science. Confocal fluorescence microscopy (CFM) facilitates high-contrast 2D and 3D images of biological samples such as living cells, and frozen or fixed tissue sections. However, to date, imaging with existing confocal microscopes has been limited by practicality, especially when samples are large and overfill the field of view (FOV) of typical microscope objectives (e.g., ~10 mm2 tissue section). In this case, image-tiling must be employed to cover the entire specimen. This can be time consuming and cause artifacts in the composite image. The MACROscope® system (Biomedical Photometrics Inc, Waterloo, Canada), is a confocal device with a 2x7 cm2 FOV, and is ideal for imaging large tissue sections in a single frame. The system used in this work is a prototype capable of simultaneous acquisition from two detection channels. Reflected light (RL), transmitted light (TL) and differential phase contrast (DPC) images of whole cut mouse tumor xenografts were collected with the same system. Preliminary results demonstrate that the MACROscope® can produce high quality images of large tissue samples; comparable in resolution and contrast to those obtained with conventional CFM using low-power (5-10x) objectives, but at increased imaging speeds (>10x), and FOV (>20x). This new device avoids the need for image-tiling and provides simultaneous imaging of multiple tissue-specific fluorescent labels in large biological samples with high resolution and contrast; thereby allowing time- and cost-efficient high-throughput screening of immunohistopathological samples. This device may also serve in the imaging of high-throughput DNA and tissue arrays.

Paper Details

Date Published: 9 December 2004
PDF: 10 pages
Proc. SPIE 5578, Photonics North 2004: Photonic Applications in Astronomy, Biomedicine, Imaging, Materials Processing, and Education, (9 December 2004); doi: 10.1117/12.567467
Show Author Affiliations
Paul Constantinou, Univ. of Toronto (Canada)
Trudey Nicklee, Princess Margaret Hospital (Canada)
David W. Hedley, Princess Margaret Hospital (Canada)
Brian C. Wilson, Univ. of Toronto (Canada)
Savvas Damaskinos, Biomedical Photometrics Inc. (Canada)

Published in SPIE Proceedings Vol. 5578:
Photonics North 2004: Photonic Applications in Astronomy, Biomedicine, Imaging, Materials Processing, and Education
Marc Nantel; Glen Herriot; Graham H. McKinnon; Leonard MacEachern; Robert A. Weersink; Rejean Munger; Andrew Ridsdale, Editor(s)

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