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Proceedings Paper

Nanoparticle-labeled DNA capture elements for detection and identification of biological agents
Author(s): Johnathan L. Kiel D.V.M.; Eric A. Holwitt; Jill E. Parker; Jeevalatha Vivekananda; Veronica Franz
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Paper Abstract

Aptamers, synthetic DNA capture elements (DCEs), can be made chemically or in genetically engineered bacteria. DNA capture elements are artificial DNA sequences, from a random pool of sequences, selected for their specific binding to potential biological warfare or terrorism agents. These sequences were selected by an affinity method using filters to which the target agent was attached and the DNA isolated and amplified by polymerase chain reaction (PCR) in an iterative, increasingly stringent, process. The probes can then be conjugated to Quantum Dots and super paramagnetic nanoparticles. The former provide intense, bleach-resistant fluorescent detection of bioagent and the latter provide a means to collect the bioagents with a magnet. The fluorescence can be detected in a flow cytometer, in a fluorescence plate reader, or with a fluorescence microscope. To date, we have made DCEs to Bacillus anthracis spores, Shiga toxin, Venezuelan Equine Encephalitis (VEE) virus, and Francisella tularensis. DCEs can easily distinguish Bacillus anthracis from its nearest relatives, Bacillus cereus and Bacillus thuringiensis. Development of a high through-put process is currently being investigated.

Paper Details

Date Published: 29 December 2004
PDF: 6 pages
Proc. SPIE 5617, Optically Based Biological and Chemical Sensing for Defence, (29 December 2004); doi: 10.1117/12.566640
Show Author Affiliations
Johnathan L. Kiel D.V.M., Air Force Research Lab. (United States)
Eric A. Holwitt, Air Force Research Lab. (United States)
Jill E. Parker, Air Force Research Lab. (United States)
Jeevalatha Vivekananda, Conceptual MindWorks, Inc. (United States)
Veronica Franz, Conceptual MindWorks, Inc. (United States)

Published in SPIE Proceedings Vol. 5617:
Optically Based Biological and Chemical Sensing for Defence
John C. Carrano; Arturas Zukauskas, Editor(s)

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