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Proceedings Paper

Toward fluorescence detection of protein residues on surgical instruments
Author(s): Patricia R. Richardson; Anita C. Jones; Robert L. Baxter; Helen C. Baxter; A. Gavin Whittaker; Gaynor A. Campbell
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Paper Abstract

Prion proteins are the infectious agents that cause Creutzfeldt-Jakob Disease (CJD) in humans. These proteins are particularly resistant to normal sterilization procedures, and the theoretical risk of prion transmission via surgical instruments is of current public and professional concern. We are currently investigating fluorescence methods for the detection of proteins on surfaces, with a view to developing an optical-fiber-based system for routine, online monitoring of residual protein contamination on surgical instruments, in hospital sterilization departments. This paper presents preliminary results on the detection of femtomole amounts of fluorescently labelled protein on surgical steel and discusses some of the problems involved in the detection of fluorescence from metal samples.

Paper Details

Date Published: 9 June 2004
PDF: 4 pages
Proc. SPIE 5502, Second European Workshop on Optical Fibre Sensors, (9 June 2004); doi: 10.1117/12.566604
Show Author Affiliations
Patricia R. Richardson, Univ. of Edinburgh (United Kingdom)
Anita C. Jones, Univ. of Edinburgh (United Kingdom)
Robert L. Baxter, Univ. of Edinburgh (United Kingdom)
Helen C. Baxter, Univ. of Edinburgh (United Kingdom)
A. Gavin Whittaker, Univ. of Edinburgh (United Kingdom)
Gaynor A. Campbell, Univ. of Edinburgh (United Kingdom)

Published in SPIE Proceedings Vol. 5502:
Second European Workshop on Optical Fibre Sensors
Jose Miguel Lopez-Higuera; Brian Culshaw, Editor(s)

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