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Proceedings Paper

Theoretical considerations for evanescent-wave immunosensors in biomedical applications
Author(s): Donna S. Orvedahl; Walter F. Love; Rudolf E. Slovacek
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Paper Abstract

Using evanescent wave immunosensors, a fluorescent labeled analyte may be concentrated within the active surface region by a combination of diffusion to the fiber surface and trapping of the molecule or complex by an antigen-antibody affinity reaction. With the dye B- phycoerythrin, approximately 1.5 X 10-22 moles may be sensed over a 1 mm2 surface. From this number (as determined for a particular dye and instrumentation system) and antibody affinity constants, limits to assay sensitivity can be calculated and the kinetics modeled.

Paper Details

Date Published: 1 March 1992
PDF: 12 pages
Proc. SPIE 1587, Chemical, Biochemical, and Environmental Fiber Sensors III, (1 March 1992); doi: 10.1117/12.56545
Show Author Affiliations
Donna S. Orvedahl, Ciba Corning Diagnostic Corp. (United States)
Walter F. Love, Corning Incorporated (United States)
Rudolf E. Slovacek, Ciba Corning Diagnostics Corp. (United States)

Published in SPIE Proceedings Vol. 1587:
Chemical, Biochemical, and Environmental Fiber Sensors III
Robert A. Lieberman, Editor(s)

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