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Proceedings Paper

Effect of ionic strength on electrically evoked membrane tether force: an optical tweezers study
Author(s): Feng Qian; William E. Brownell; Bahman Anvari
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Paper Abstract

We have investigated the effect of ionic strength on electrically evoked membrane tether force using optical tweezers. Membrane tethers from cochlear outer hair cells (OHCs) and human embryonic kidney (HEK) cells exhibited mechanical response to applied voltage stimuli over a wide frequency range. The electrically evoked variations in the tether force were probed by an optically-trapped microsphere, the image of which was projected on a quadrant photodiode for dynamic measurement of its displacements. Compared to normal saline (140mM NaCl), low ionic-strength solution (10mM NaCl) blocked the electrically evoked tether force for both OHCs and HEK cells. As the Debye length for membrane bilayer was estimated to increase from approximately 0.75nm to 1.88nm, the internal repulsive pressure of the membrane tethers rose consequently, resulting in the enlargement in the equilibrium tether diameter and the decrease in the tether force.

Paper Details

Date Published: 18 October 2004
PDF: 6 pages
Proc. SPIE 5514, Optical Trapping and Optical Micromanipulation, (18 October 2004);
Show Author Affiliations
Feng Qian, Rice Univ. (United States)
William E. Brownell, Baylor College of Medicine (United States)
Bahman Anvari, Rice Univ. (United States)

Published in SPIE Proceedings Vol. 5514:
Optical Trapping and Optical Micromanipulation
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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