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Proceedings Paper

A double trap for single-molecule manipulation at opaque and microstructured surfaces
Author(s): Olivier Legrand; Denis Cote; Ulrich Bockelmann
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Paper Abstract

We describe a double optical trap for micromanipulation of biomolecules. This set-up allows to bring a DNA molecule grafted between two beads in contact with microelectrodes, in order to perform electrical measurements at the single molecule level in a well-controlled geometry. The use of an opaque surface like silicon compatible with lithography imposes to work in a reflection configuration with the same objective for trapping and imaging the bead. The experimental constraints raised by this optical configuration are detailed. By combining drag force and optical trapping, we are able to trap separately the two beads and to perform force versus extension measurements in this reflection configuration. Finally, deposition of one stretched DNA molecule on Au electrodes fabricated by electron beam lithography on silicon wafers is shown.

Paper Details

Date Published: 18 October 2004
PDF: 10 pages
Proc. SPIE 5514, Optical Trapping and Optical Micromanipulation, (18 October 2004); doi: 10.1117/12.559795
Show Author Affiliations
Olivier Legrand, Ecole Normale Superieure (France)
Ctr. de Recherche Motorola (France)
Denis Cote, Ecole Normale Superieure (France)
Ulrich Bockelmann, Ecole Normale Superieure (France)

Published in SPIE Proceedings Vol. 5514:
Optical Trapping and Optical Micromanipulation
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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