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Proceedings Paper

Optical probing of specific and nonspecific interactions with nanometer resolution
Author(s): Jakob Kisbye Dreyer; Poul Martin Hansen; Lene Broeng Oddershede
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Paper Abstract

Using optical tweezers combined with video based methods we probe non-specific interactions between polystyrene beads and surfaces at different electrolyte concentrations. We present force-distance relationships and using statiscally based maximum likelihood methods, which significantly improves spatial resolutions, the interaction potentials are also found. At low electrolyte concentrations the interaction potentials are in agreement with DLVO theory, but at high electrolyte concentrations the interaction potentials deviate both from DVLE and Lifshitz theories. Also, we probe the specific interaction between biotin and streptavidin. However, these measurements are complicated because the interaction is of very short range and a streptavidin coated polystyrene bead adheres to a biotin coated surface as soon as it encounters the surface during its Brownian motion in the trap.

Paper Details

Date Published: 18 October 2004
PDF: 8 pages
Proc. SPIE 5514, Optical Trapping and Optical Micromanipulation, (18 October 2004); doi: 10.1117/12.559622
Show Author Affiliations
Jakob Kisbye Dreyer, Niels Bohr Institute (Denmark)
Poul Martin Hansen, Niels Bohr Institute (Denmark)
Lene Broeng Oddershede, Niels Bohr Institute (Denmark)

Published in SPIE Proceedings Vol. 5514:
Optical Trapping and Optical Micromanipulation
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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