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Proceedings Paper

Study of DNA deformation under flow using optical tweezers
Author(s): Eirini Theofanidou; Tsueu Ju Su; Jochen Arlt; David T. F. Dryden; William J. Hossack; Jason Crain; Wilson C. K. Poon
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Paper Abstract

The stretching and unwinding of polymers under flow is important for understanding the rheological properties of dilute polymer solutions. Scaling theory based on the blob picture of single polymer chains predicts several regimes for the overall shape of a hydrodynamically deformed macromolecule. We studied the shape of a DNA molecule stretched out by steady uniform flow at different velocities using optical trapping of single DNA molecules (tethered on polystyrene beads) and single molecule fluorescence imaging. The results show a gradual transition from a non-draining regime at low velocities to a free-draining regime at high velocities, thus verifying the predictions of the free draining-shell (F-shell) blob model.

Paper Details

Date Published: 18 October 2004
PDF: 12 pages
Proc. SPIE 5514, Optical Trapping and Optical Micromanipulation, (18 October 2004); doi: 10.1117/12.558906
Show Author Affiliations
Eirini Theofanidou, Univ. of Edinburgh (United Kingdom)
Tsueu Ju Su, Univ. of Edinburgh (United Kingdom)
Jochen Arlt, Univ. of Edinburgh (United Kingdom)
David T. F. Dryden, Univ. of Edinburgh (United Kingdom)
William J. Hossack, Univ. of Edinburgh (United Kingdom)
Jason Crain, Univ. of Edinburgh (United Kingdom)
Wilson C. K. Poon, Univ. of Edinburgh (United Kingdom)

Published in SPIE Proceedings Vol. 5514:
Optical Trapping and Optical Micromanipulation
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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