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Proceedings Paper

On the fundamental imaging-depth limit in two-photon microscopy
Author(s): Patrick Theer; Winfried T. Denk
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Paper Abstract

The limit on imaging-depth in two-photon microscopy depends on parameters of the imaging system such as available power, wavelength, numerical aperture, and fluorescencecollection field-of-view and on properties of the sample such as scattering and absorption cross-sections and fluorophore distribution. These dependencies are discussed and strategies for optimizing the imaging depth are presented.

Paper Details

Date Published: 8 September 2004
PDF: 11 pages
Proc. SPIE 5463, Femtosecond Laser Applications in Biology, (8 September 2004); doi: 10.1117/12.548057
Show Author Affiliations
Patrick Theer, Max-Planck Institute for Medical Research (Germany)
Winfried T. Denk, Max-Planck Institute for Medical Research (Germany)

Published in SPIE Proceedings Vol. 5463:
Femtosecond Laser Applications in Biology
Sigrid Avrillier; Jean-Michel Tualle, Editor(s)

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