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Proceedings Paper

Application of confocal microscopy in neurons imaging
Author(s): Xiaoxiang Zheng
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Paper Abstract

Nitric oxide (NO) and calcium ions (Ca2+) play critical role as molecular mediator in many physiological processes. However, their low concentration and instability in specimen make them difficult to be detected directly in neurons. We developed a method for imaging nitric oxide and calcium ions using Laser Scanning Confocal Microscopy (LSCM). Cultured hippocampal neuron is dyed and observed under Zeiss LSM 510 laser scanning confocal microscope. Excited by laser the emission light from the labeled nitric oxide and calcium ions in the neutron are detected. In this way, the nitric oxide and calcium ions are imaged and their intracellular kinetic change in monitored. Furthermore, image processing and visualization techniques are employed to help analyze the image data.

Paper Details

Date Published: 4 December 2003
PDF: 7 pages
Proc. SPIE 5255, Biomolecular Photonics and Multidimensional Microscopy, (4 December 2003); doi: 10.1117/12.546237
Show Author Affiliations
Xiaoxiang Zheng, Zhejiang Univ. (China)

Published in SPIE Proceedings Vol. 5255:
Biomolecular Photonics and Multidimensional Microscopy
Qingming Luo; Min Gu, Editor(s)

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