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Proceedings Paper

H-bond sensing with 3-hydroxyflavones: steady-state and time-resolved fluorescence studies
Author(s): Vasyl V. Shynkar; Andrey S. Klymchenko; Alexander P. Demchenko; Yves Mely
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Paper Abstract

The 4'-dialkylamino derivatives of 3-hydroxyflavone find many applications as molecular probes, since their two-band fluorescence spectra produce a strong response to different intermolecular interactions including H-bonding. The results of our steady-state and time-resolved studies in neat and mixed solvents reveal an important and probably unique property of these dyes: their ground-state equilibrium between H-bonded and non H-bonded forms is not changed significantly on excitation to the normal (N*) excited state. In the excited state, new H-bonds do not form but those already existing in the ground state can disrupt on a slow time scale. This last process is probably coupled with the slow excited-state intramolecular proton transfer (ESIPT) reaction of the H-bonded form of the dye. These dyes do not change significantly the distribution between H-bonded and non H-bonded species in their environment and therefore they can provide a measure of the H-bonding potential of their environment. Due to this feature, they can serve as unique sensors of the H-bonding potential in unknown media. This sensing can be provided by the dramatic change of the relative intensities of their two separated emission bands.

Paper Details

Date Published: 1 September 2004
PDF: 11 pages
Proc. SPIE 5459, Optical Sensing, (1 September 2004); doi: 10.1117/12.545573
Show Author Affiliations
Vasyl V. Shynkar, Univ. Louis Pasteur (France)
Kyiv National Taras Shevchenko Univ. (Ukraine)
Andrey S. Klymchenko, Univ. Louis Pasteur (France)
TUBITAK Marmara Research Ctr. (Turkey)
Alexander P. Demchenko, TUBITAK Marmara Research Ctr. (Turkey)
A.V. Palladin Institute of Biochemistry (Ukraine)
Yves Mely, Univ. Louis Pasteur (France)

Published in SPIE Proceedings Vol. 5459:
Optical Sensing
Brian Culshaw; Anna Grazia Mignani; Rainer Riesenberg, Editor(s)

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