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Proceedings Paper

High-resolution multiphoton tomography of human skin in vivo and in vitro
Author(s): Iris Riemann; Enrico Dimitrov; Peter Fischer; Annette Reif; Martin Kaatz; Peter Elsner; Karsten Konig
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Paper Abstract

The novel compact femtosecond NIR (near infrared) laser imaging system DermaInspect was used to perform for the first time in vivo high resolution non-invasive 4D tomography of human skin based on multiphoton autofluorescence imaging and second harmonic generation (SHG). Using fast galvoscan mirrors, a time correlated single photon counting (TCSPC) module and femtosecond 80 MHz laser pulses in the spectral range of 750 nm-850 nm human skin was analyzed with subcellular spatial resolution (3D) and 250 ps temporal resolution (4D). The non-linear induced autofluorescence originates from naturally endogenous fluorophores and protein structures like NAD(P)H, flavins, phorphyrins, melanin, elastin and collagen. Collagenous structures were detected using SHG. Tissues of patients with dermatological disorders like nevi and melanoma have been investigated with a clear visualization of cells and intratissue structures. Further characterization of those components was performed by the fluorescence lifetime imaging (FLIM) and the determination of two photon excitation spectra. This method of non invasive high resolution optical biopsy provides a painless diagnostic tool for dermatological applications.

Paper Details

Date Published: 8 September 2004
PDF: 8 pages
Proc. SPIE 5463, Femtosecond Laser Applications in Biology, (8 September 2004); doi: 10.1117/12.545561
Show Author Affiliations
Iris Riemann, Fraunhofer Institute for Biomedical Technology (Germany)
Enrico Dimitrov, Friedrich-Schiller-Univ. Jena (Germany)
Peter Fischer, JenLab GmbH (Germany)
Annette Reif, Friedrich-Schiller-Univ. Jena (Germany)
Martin Kaatz, Friedrich-Schiller-Univ. Jena (Germany)
Peter Elsner, Friedrich-Schiller-Univ. Jena (Germany)
Karsten Konig, Fraunhofer Institute for Biomedical Technology (Germany)

Published in SPIE Proceedings Vol. 5463:
Femtosecond Laser Applications in Biology
Sigrid Avrillier; Jean-Michel Tualle, Editor(s)

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