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Proceedings Paper

Reducing and shaping the fluorescence detection volume for single bio-molecules analysis: the assets of nanophotonic structures
Author(s): Herve Rigneault; Emilien Etienne; Pierre-Francois Lenne
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Paper Abstract

We review and discuss recent concepts and methods used to detect single fluorescent molecules with sub-wavelength resolution for biological analysis. We start with a brief review of the far field techniques that are able to define an observation volume, which is of the order or smaller than the optical diffraction limit. In a second part, we discuss how photonics structures can be used to shape both the excitation and the emitted optical fields, leading to a better signal to noise ratio and an enhancement of the temporal and spatial resolutions. We show that this 'nano-optical field engineering' can be associated with single molecule detection techniques such as Fluorescence Correlation Spectroscopy. In this framework, we illustrate the potentialities of planar photonic structures for the study of confined molecular diffusion in cells and bacteria.

Paper Details

Date Published: 15 September 2004
PDF: 10 pages
Proc. SPIE 5450, Photonic Crystal Materials and Nanostructures, (15 September 2004); doi: 10.1117/12.545087
Show Author Affiliations
Herve Rigneault, Institut Fresnel, Univ. d'Aix-Marseille III (France)
Emilien Etienne, Institut Fresnel, Univ. d'Aix-Marseille III (France)
Pierre-Francois Lenne, Institut Fresnel, Univ. d'Aix-Marseille III (France)

Published in SPIE Proceedings Vol. 5450:
Photonic Crystal Materials and Nanostructures
Richard M. De La Rue; Pierre Viktorovitch; Clivia M. Sotomayor Torres; Michele Midrio, Editor(s)

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