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Proceedings Paper

Three-dimensional reconstruction of large tissue volumes from scanning laser confocal microscopy
Author(s): Mohamed-Adel Slamani; Andrzej Krol; Jacques Beaumont; Robert L. Price; Ioana L. Coman; Edward D. Lipson
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Paper Abstract

Phase correlation is applied to the mosaicing of confocal scanning laser microscopy data. A large specimen (i.e., a murine heart) is cut into a number of individual sections with appropriate thickness. The sections are scanned horizontally and vertically to produce tiles of a 3D volume. Image processing based on phase correlation is used to rebuild the 3D volume and stitch the tiles together. Specifically, 2D registration of in-plane tiles and 3D alignment of optical slices within a given physical section are performed. The approach and performance are presented in this paper along with examples.

Paper Details

Date Published: 12 May 2004
PDF: 8 pages
Proc. SPIE 5370, Medical Imaging 2004: Image Processing, (12 May 2004); doi: 10.1117/12.536232
Show Author Affiliations
Mohamed-Adel Slamani, ITT Industries, Inc. (United States)
Andrzej Krol, SUNY Upstate Medical Ctr. (United States)
Jacques Beaumont, SUNY Upstate Medical Ctr. (United States)
Robert L. Price, Univ. of South Carolina School of Medicine (United States)
Ioana L. Coman, Ithaca College (United States)
Edward D. Lipson, Syracuse Univ. (United States)

Published in SPIE Proceedings Vol. 5370:
Medical Imaging 2004: Image Processing
J. Michael Fitzpatrick; Milan Sonka, Editor(s)

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