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Proceedings Paper

DNA capture elements for rapid detection and identification of biological agents
Author(s): Johnathan L. Kiel D.V.M.; Jill E. Parker; Eric A. Holwitt; Jeeva Vivekananda
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Paper Abstract

DNA capture elements (DCEs; aptamers) are artificial DNA sequences, from a random pool of sequences, selected for their specific binding to potential biological warfare agents. These sequences were selected by an affinity method using filters to which the target agent was attached and the DNA isolated and amplified by polymerase chain reaction (PCR) in an iterative, increasingly stringent, process. Reporter molecules were attached to the finished sequences. To date, we have made DCEs to Bacillus anthracis spores, Shiga toxin, Venezuelan Equine Encephalitis (VEE) virus, and Francisella tularensis. These DCEs have demonstrated specificity and sensitivity equal to or better than antibody.

Paper Details

Date Published: 13 August 2004
PDF: 6 pages
Proc. SPIE 5416, Chemical and Biological Sensing V, (13 August 2004); doi: 10.1117/12.532043
Show Author Affiliations
Johnathan L. Kiel D.V.M., Air Force Research Lab. (United States)
Jill E. Parker, Air Force Research Lab. (United States)
Eric A. Holwitt, Air Force Research Lab. (United States)
Jeeva Vivekananda, Conceptual Mindworks, Inc. (United States)

Published in SPIE Proceedings Vol. 5416:
Chemical and Biological Sensing V
Patrick J. Gardner, Editor(s)

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