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Proceedings Paper

Microfabricated arrays of elastomeric posts to study cellular mechanics
Author(s): Olivia du Roure; Caroline Dequidt; Alain Richert; Robert H. Austin; Axel Buguin; Philippe Chavrier; Pascal Silberzan; Benoit Ladoux
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Paper Abstract

We present an approach to fabricate an array of elastomer posts in order to dynamically measure the traction forces exerted by living cells on a surface with a micrometer lateral resolution. Arrays of closely spaced vertical microposts are made in silicone elastomer [poly(dimethylsiloxane) (PDMS)] by molding a Silicon substrate that has been machined by deep Si etching after standard photolithography. The surface of the micropillars was modified to allow cell culture. Deflections of the calibrated posts were dynamically followed by direct obervation with an optical microscope. By using this set-up, we could dynamically draw up a cartography of the local traction forces exerted by the cells.

Paper Details

Date Published: 23 December 2003
PDF: 9 pages
Proc. SPIE 5345, Microfluidics, BioMEMS, and Medical Microsystems II, (23 December 2003); doi: 10.1117/12.530688
Show Author Affiliations
Olivia du Roure, Univ. Paris 7 Denis-Diderot (France)
Caroline Dequidt, Univ. Paris 7 Denis-Diderot (France)
Alain Richert, Univ. Paris 7 Denis-Diderot (France)
Robert H. Austin, Princeton Univ. (United States)
Axel Buguin, Institut Curie (France)
Philippe Chavrier, Institut Curie (France)
Pascal Silberzan, Institut Curie (France)
Benoit Ladoux, Univ. Paris 7 Denis-Diderot (France)

Published in SPIE Proceedings Vol. 5345:
Microfluidics, BioMEMS, and Medical Microsystems II
Peter Woias; Ian Papautsky, Editor(s)

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