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Proceedings Paper

Cancerigens or radiation action on chromatin determined by spectrofluorimetric methods
Author(s): Liliana Radu; Doina M. Gazdaru; O. Horer; B. Constantinescu; Ion N. Mihailescu
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Paper Abstract

Steady state and time resolved fluorescence and fluorescence resonance energy transfer (FRET) methods were used in the determination of cancerigens or radiation action on chromatin -- the complex of deoxyribonucleic acid (DNA) with proteins, that exists in eukaryotic cells nuclei. The results indicated modifications in general chromatin structure, DNA strand breaks with the reduction of the double helix DNA proportion (for radiation action) or the growth of the proportion of available DNA for ligand binding (in cancerigens action), proteins structure destruction and an increase of acidic proteins proportion on cancerigens action. The action of other physical or chemical agents may be analyzed by these high performance optical methods.

Paper Details

Date Published: 19 November 2003
PDF: 2 pages
Proc. SPIE 4829, 19th Congress of the International Commission for Optics: Optics for the Quality of Life, (19 November 2003); doi: 10.1117/12.530486
Show Author Affiliations
Liliana Radu, Victor Babes Institute (Romania)
Doina M. Gazdaru, Univ. of Bucharest (Romania)
O. Horer, S. Nicolau Institute (Romania)
B. Constantinescu, Institute of Atomic Physics (Romania)
Ion N. Mihailescu, Institute of Atomic Physics (Romania)

Published in SPIE Proceedings Vol. 4829:
19th Congress of the International Commission for Optics: Optics for the Quality of Life
Giancarlo C. Righini; Anna Consortini, Editor(s)

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