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Proceedings Paper

Fourier-transform phase-modulation fluorometer
Author(s): Tetsuo Iwata; Takeshi Kamada; Tsutomu Araki
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Paper Abstract

We propose a novel phase-modulation fluorometer by which fluorescence decay waveform can be obtained. In the fluorometer, the modulation frequency of the excitation light source is varied from fmin to fmax continuously within a time interval of T. The resultant sinusoidal fluorescence waveform is Fourier-transformed to obtain the amplitude and phase spectra. The ratio of the amplitude spectrum and the difference of the phase spectrum over those of the reference spectra that are obtained from a non-fluorescent material are calculated, respectively, and the data are inverse-Fourier-transformed to obtain the fluorescence decay waveform. We carried out numerical simulations and fundamental experiments using an electric RC differential circuit and a white light-emitting diode (LED). We call the method Fourier-transform phase-modulation fluorometry.

Paper Details

Date Published: 19 November 2003
PDF: 2 pages
Proc. SPIE 4829, 19th Congress of the International Commission for Optics: Optics for the Quality of Life, (19 November 2003); doi: 10.1117/12.530349
Show Author Affiliations
Tetsuo Iwata, Univ. of Tokushima (Japan)
Takeshi Kamada, Univ. of Tokushima (Japan)
Tsutomu Araki, Osaka Univ. (Japan)

Published in SPIE Proceedings Vol. 4829:
19th Congress of the International Commission for Optics: Optics for the Quality of Life
Giancarlo C. Righini; Anna Consortini, Editor(s)

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