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Proceedings Paper

Lanthanide-enhanced luminescence (LEL) with one and two photon excitation of Quantum Dyes lanthanide(III)-macrocycles
Author(s): Robert C. Leif; Margie C. Becker; Alfred J. Bromm Jr.; Nanguang Chen; Ann E. Cowan; Lidia M. Vallarino; Sean Yang; Robert M. Zucker
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Paper Abstract

Improvements in the lanthanide enhanced luminescence (LEL) protocol have facilitated the use of the recently synthesized Eu(III)-macrocycle-mono-isothiocyanate, Quantum Dye, as a label. It was discovered that a homogeneous solution in ethanol or other solvent could be used to produce the lanthanide enhanced luminescence (LEL) effect, provided that the solution was permitted to evaporate. This protocol has been applied to the direct staining of cells in S phase, and was optimized for solid phase assays with Quantum Dye labeled streptavidin. Preliminary studies indicate that cells stained with the europium Quantum Dye can be observed both by conventional UV laser excitation and by infrared two-photon confocal microscopy. An enhancer has been found that enables the observation of simultaneous emissions from both the europium and terbium Quantum Dyes.

Paper Details

Date Published: 1 July 2004
PDF: 13 pages
Proc. SPIE 5322, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues II, (1 July 2004); doi: 10.1117/12.530284
Show Author Affiliations
Robert C. Leif, Newport Instruments (United States)
Margie C. Becker, Phoenix Flow Systems (United States)
Alfred J. Bromm Jr., Virginia Commonwealth Univ. (United States)
Nanguang Chen, Univ. of Connecticut (United States)
Ann E. Cowan, Health Ctr./Univ. of Connecticut (United States)
Lidia M. Vallarino, Virginia Commonwealth Univ. (United States)
Sean Yang, Newport Instruments (United States)
Phoenix Flow Systems (United States)
Robert M. Zucker, U.S. Environmental Protection Agency (United States)

Published in SPIE Proceedings Vol. 5322:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues II
Dan V. Nicolau; Joerg Enderlein; Robert C. Leif; Daniel L. Farkas, Editor(s)

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