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Proceedings Paper

Metal-enhanced fluorescence: a novel approach to ultra-sensitive fluorescence sensing assay platforms
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Paper Abstract

We describe the development of a novel generic approach to fluorescence sensing based on metal-enhanced fluorescence (MEF). This work follows our initial reports of radiative decay engineering (RDE), where we experimentally demonstrated dramatic signal enhancements of fluorophores positioned close to surface-bound silver nanostructures. The attractive changes in spectral properties of fluorophores includes increased rates of excitation, increased quantum yields, decreased fluorescence lifetimes with an increased photostability, and drastically increased rates of multi-photon excitation. In this report we present a new class of fluorescent biomarkers which are strongly enhanced by metallic particles. This has afforded the development of a novel generic approach for ultra-sensitive fluorescence assay technology. The assay platform utilizes metal particles deposited on glass/quartz surfaces, covered with sub-nanometer layers of a fluorescent biomarker. As such the fluorescence signal of the composite is strongly enhanced. This readily allows easy, quantitative and inexpensive fluorescence detection of minimal traces of specific antigens. We also explore different sensing geometries, such as using evanescent wave excitation.

Paper Details

Date Published: 1 July 2004
PDF: 8 pages
Proc. SPIE 5321, Biomedical Vibrational Spectroscopy and Biohazard Detection Technologies, (1 July 2004); doi: 10.1117/12.530072
Show Author Affiliations
Zygmunt Gryczynski, Univ. of Maryland/Baltimore (United States)
Joanna Malicka, Univ. of Maryland/Baltimore (United States)
Ignacy Gryczynski, Univ. of Maryland/Baltimore (United States)
Evgenia Matveeva, Univ. of Maryland/Baltimore (United States)
Chris D. Geddes, Univ. of Maryland/Baltimore (United States)
Kadir Aslan, Univ. of Maryland/Baltimore (United States)
Joseph R. Lakowicz, Univ. of Maryland/Baltimore (United States)

Published in SPIE Proceedings Vol. 5321:
Biomedical Vibrational Spectroscopy and Biohazard Detection Technologies
Zygmunt Gryczynski; Anita Mahadevan-Jansen; Michael G. Sowa; Tuan Vo-Dinh; Joseph R. Lakowicz; Gerwin J. Puppels, Editor(s)

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