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Proceedings Paper

Performance of dual axes confocal microscope for in vivo molecular and cellular imaging
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Paper Abstract

The application of conventional confocal microscopes with high numerical aperture (NA) to in vivo imaging is limited by the objectiveís large physical dimensions and short working distance. We are developing a confocal microscope that uses simple low NA lenses oriented in a dual axes configuration for miniaturization and in vivo imaging. This architecture achieves a long working distance, micron level axial resolution, and reduced noise from scattered light outside the focal volume. Combined with the novel method of post-objective scanning, this design can be scaled down to millimeter dimensions. We derive the dual axes response from diffraction theory, and construct two tabletop prototypes to demonstrate the performance of this approach. We collect images from freshly excised biopsy specimens of human esophagus and transgenic mouse cerebellum expressing GFP. With horizontal cross-sectional images, we achieve 1 to 2 μm resolution and collect reflectance and fluorescence images. With vertical cross-sectional images, we achieve 4 to 5 μm resolution, dynamic range of 70 dB, and tissue penetration over 1 mm. An instrument miniaturized with this configuration could be used for in vivo cellular and molecular imaging.

Paper Details

Date Published: 13 July 2004
PDF: 12 pages
Proc. SPIE 5324, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XI, (13 July 2004); doi: 10.1117/12.530016
Show Author Affiliations
Gordon S. Kino, Stanford Univ. (United States)
Thomas D. Wang M.D., Stanford Univ. (United States)
Chris H. Contag, Stanford Univ. (United States)
Michael Mandella, Optical Biopsy Technologies, Inc. (United States)
Ning Y. Chan, Optical Biopsy Technologies, Inc. (United States)

Published in SPIE Proceedings Vol. 5324:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XI
Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson, Editor(s)

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