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Proceedings Paper

Study of localization of response to fibroblast growth factor-2 in murine calvaria using Raman spectroscopic imaging
Author(s): Nicole J. Crane; Geng Geng Yu; Michael A. Ignelzi Jr.; Michael D. Morris
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Paper Abstract

Raman spectral imaging provides the means to study spatially localized response to controlled external perturbations to tissue specimens with light microscopy resolution. We discuss use of heparin-acrylic microbeads soaked with the protein fibroblast growth factor-2 (FGF2). Microbeads containing FGF2 are placed in murine calvarial tissue and stimulate abnormally rapid mineralization. The tissue response simulated the effects of craniosynostosis, a birth defect occurring in 1 in 2400 live births. We describe Raman imaging measurements of the spatial distribution of apatitic mineral and matrix (predominantly type I collagen) from normal murine calvarial tissue and murine calvarial tissue modeling craniosynostosis. We also discuss spectroscopic evaluation of the state of the mineral induced by the FGF2 beads.

Paper Details

Date Published: 1 July 2004
PDF: 8 pages
Proc. SPIE 5321, Biomedical Vibrational Spectroscopy and Biohazard Detection Technologies, (1 July 2004);
Show Author Affiliations
Nicole J. Crane, Univ. of Michigan (United States)
Geng Geng Yu, Univ. of Michigan (United States)
Michael A. Ignelzi Jr., Univ. of Michigan (United States)
Michael D. Morris, Univ. of Michigan (United States)

Published in SPIE Proceedings Vol. 5321:
Biomedical Vibrational Spectroscopy and Biohazard Detection Technologies
Zygmunt Gryczynski; Anita Mahadevan-Jansen; Michael G. Sowa; Tuan Vo-Dinh; Joseph R. Lakowicz; Gerwin J. Puppels, Editor(s)

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