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Proceedings Paper

Simultaneous visualization of contact microscopic image and energetic charged particle tracks and its application to medicine
Author(s): Kuniaki Amemiya; Hiroyuki Takahashi; Toru Naruse; Masaharu Nakazawa; Hironobu Yanagie M.D.; Tomoyuki Hisa M.D.; Masazumi Eriguchi M.D.; Yoshinobu Nakagawa M.D.; Toshikazu Majima; Teruyoshi Kageji M.D.; Yoshinori Sakurai; Tooru Kobayashi; Teruaki Konishi; Kotaro Hieda; Nakahiro Yasuda; Koichi Ogura
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Paper Abstract

Contact microscopy, enables us to visualize the detailed internal structure of biological cells. Exposure of biological specimen mounted on X-ray or UV sensitive materials to soft X-ray or UV introduces chemical damage to the materials, and the damage distribution due to the absorption of the X-rays or the UV by the specimen reveals as relief on the surface of the material after development process. The relief can be visualized with an AFM at high resolution of ~100 nm. We have applied the contact microscopy technique to high-resolution neutron-induced alpha-autoradiography for boron imaging in boron neutron capture therapy (BNCT). In BNCT, energetic alpha/lithium particles (range ~ single cell) from boron-neutron reactions introduce lethal damage to tumor cells selectively through thermal neutron irradiation with tumor-accumulating boron compounds. To understand the mechanism of drug delivery of those boron compounds is significant to evaluate the efficacy of BNCT. In the new technique, we can visualize those alpha/lithium particle tracks as etch pits and contact X-ray/UV microscopic image of tumor cells as relief on the surface of CR-39 plastic track detectors after etching process. Achievable resolution was ~100 nm with AFM readout, so that we can perform the boron imaging at subcellular scale.

Paper Details

Date Published: 1 June 2004
PDF: 8 pages
Proc. SPIE 5331, Nanobiophotonics and Biomedical Applications, (1 June 2004); doi: 10.1117/12.528777
Show Author Affiliations
Kuniaki Amemiya, Univ. of Tokyo (Japan)
Hiroyuki Takahashi, Univ. of Tokyo (Japan)
Toru Naruse, Univ. of Tokyo (Japan)
Masaharu Nakazawa, Univ. of Tokyo (Japan)
Hironobu Yanagie M.D., Univ. of Tokyo (Japan)
Tomoyuki Hisa M.D., Univ. of Tokyo (Japan)
Masazumi Eriguchi M.D., Univ. of Tokyo (Japan)
Yoshinobu Nakagawa M.D., National Kagawa Children's Hospital (Japan)
Toshikazu Majima, National Institute of Advanced Industrial Science and Technology (Japan)
Teruyoshi Kageji M.D., Univ. of Tokushima (Japan)
Yoshinori Sakurai, Kyoto Univ. (Japan)
Tooru Kobayashi, Kyoto Univ. (Japan)
Teruaki Konishi, Rikkyo Univ. (Japan)
Kotaro Hieda, Rikkyo Univ. (Japan)
Nakahiro Yasuda, National Institute of Radiological Sciences (Japan)
Koichi Ogura, Nihon Univ. (Japan)

Published in SPIE Proceedings Vol. 5331:
Nanobiophotonics and Biomedical Applications
Alexander N. Cartwright, Editor(s)

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