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Proceedings Paper

Comparison between the fluorescence spectroscopy and the 125I albumin-labeling technique for the study of skin edema dynamics
Author(s): Milene da Silva Melo; Renato Amaro Zangaro; Antonio Guillermo Jose Balbin Villaverde; Edson Antunes; Enilton Aparecido Camargo; Rodrigo Alvaro Brandao Lopes Martins; Denise Maciel Ferreira; Marcos Tadeu Tavares Pacheco; Egberto Munin
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Paper Abstract

Skin injury caused by chemicals substances as the carrageenan produces a local inflammatory reaction involving the liberation of mediators that leads to an increase in vascular permeability and, consequently, edema formation. The vascular permeability can be evaluated by measuring the amount of some extravasating specific dyes. The Evans blue dye is recommended due to its systemic effect and non-toxicity to the organism. That dye binds to the plasma albumin and emits radiation when excited, allowing for spectroscopic monitoring of the edema. In this study, the amount of extravasating plasma albumin in the site of the carrageenan-induced edema in Wistar rats is evaluated by fluorescence spectroscopy. The intensity of the Evans blue dye fluorescence signal for different edema evolution times is compared to the 125I labeled albumin data obtained with a g-counter. A dye laser (458 nm) was used as the fluorescence excitation source. The fluorescence intensity was taken at the 680 nm peak of the dye spectral emission. The spectroscopic data shows the dye emission intensity growing with the settling up of the edema and decreasing as the tissue recovers from the inflammatory stimulus. A good correlation between the spectroscopic and the g-counter data was obtained, which suggests that the Evans blue dye fluorescence is a promising technique for the qualitative and quantitative analysis of edema dynamics.

Paper Details

Date Published: 13 July 2004
PDF: 5 pages
Proc. SPIE 5326, Optical Biopsy V, (13 July 2004); doi: 10.1117/12.528098
Show Author Affiliations
Milene da Silva Melo, Univ. do Vale do Paraiba (Brazil)
Renato Amaro Zangaro, Univ. do Vale do Paraiba (Brazil)
Antonio Guillermo Jose Balbin Villaverde, Univ. do Vale do Paraiba (Brazil)
Edson Antunes, Univ. Estadual de Campinas (Brazil)
Enilton Aparecido Camargo, Univ. Estadual de Campinas (Brazil)
Rodrigo Alvaro Brandao Lopes Martins, Univ. do Vale do Paraiba (Brazil)
Denise Maciel Ferreira, Univ. do Vale do Paraiba (Brazil)
Marcos Tadeu Tavares Pacheco, Univ. do Vale do Paraiba (Brazil)
Egberto Munin, Univ. do Vale do Paraiba (Brazil)

Published in SPIE Proceedings Vol. 5326:
Optical Biopsy V
Robert R. Alfano; Alvin Katz, Editor(s)

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