The development of a technique that gives early diagnosis and is non-invasive is of crucial importance for public health. Raman spectroscopy is a technique that can full fill these requirements. The main goal of this work was to use the FT-Raman spectroscopy to differentiate between normal skin and the squamous cell carcinoma (SCC) tissues in vitro. The samples used in this study were collected by traditional human biopsy of skin tissue. The samples removed from the patients were washed in physiologic serum and frozen in liquid nitrogen. The FT-Raman device used was the RFS 100- Bruker, with a 1064nm from the Nd:YAG as an excitation source. After the Raman measurement the samples were submitted for histopathological study for comparation. The Raman spectra in the normal tissue showed the presence of vibrational bands in 860 cm^-1 and 939 cm^-1 with higher intensity than in the carcinoma spectra. These modes were assigned to the vibration of proline and hydroxiproline. The shift region of 1555 to 1560 cm^-1 showed a difference of intensity to the samples of squamous cell carcinoma, which were attributed to the nucleic acid.

Date Published: 13 July 2004
PDF: 7 pages
Proc. SPIE 5326, Optical Biopsy V, (13 July 2004); doi: 10.1117/12.528030

Published in SPIE Proceedings Vol. 5326:
Optical Biopsy V
Robert R. Alfano; Alvin Katz, Editor(s)