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Proceedings Paper

Laser background characterization in a monolithically integrated bio-fluorescence sensor
Author(s): Evan P. Thrush; Ofer Levi; Laura J. Cook; Jason Deich; Stephen J. Smith; W. E. Moerner; James S. Harris Jr.
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Paper Abstract

Miniaturized and robust sensing modules are required for the development of portable integrated biological analysis systems or micro total analysis systems (μ-TAS). This work uses vertical cavity surface emitting lasers (VCSELs), optical emission filters and PIN photodetectors to realize a monolithically integrated, near-infrared, fluorescence detection system. The integration of these optoelectronic devices with biochips will drastically reduce cost of current systems and increase parallelism and portability. The sensor has been implemented on a micro-fluidic format, and sensitivity was evaluated. A theoretical limit of detection of IR-800 dye in methanol is reported to be 40 nM. The sensor sensitivity is limited by laser background as a result of integrating the optoelectronic elements in such close proximity. Significant reduction in laser background from reflections above the sensor is possible by increasing the distance between the sensor and optical interfaces to greater than 3 mm. Also, for distances greater than 500 microns between the sensor and optical interfaces above the sensor, it is found that background from indirect spontaneous emission is much smaller than the background caused by specular reflections of the laser.

Paper Details

Date Published: 1 July 2004
PDF: 7 pages
Proc. SPIE 5318, Advanced Biomedical and Clinical Diagnostic Systems II, (1 July 2004); doi: 10.1117/12.525133
Show Author Affiliations
Evan P. Thrush, Stanford Univ. (United States)
Ofer Levi, Stanford Univ. (United States)
Laura J. Cook, Stanford Univ. (United States)
Jason Deich, Stanford Univ. (United States)
Stephen J. Smith, Stanford Univ. (United States)
W. E. Moerner, Stanford Univ. (United States)
James S. Harris Jr., Stanford Univ. (United States)

Published in SPIE Proceedings Vol. 5318:
Advanced Biomedical and Clinical Diagnostic Systems II
Gerald E. Cohn; Warren S. Grundfest M.D.; David A. Benaron M.D.; Tuan Vo-Dinh, Editor(s)

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