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Proceedings Paper

Investigation of doxorubicin for multidrug resistance using a fluorescent cytometric imaging system integrated onto cell culture analog devices
Author(s): Donghyun Kim; Hui Xu; Sung June Kim; Michael L. Shuler
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Paper Abstract

An integrated cytometric fluorescent imaging system is developed for characterizing chemical concentration and cellular status in microscale cell culture analog (μCCA) devices. A μCCA is used to evaluate the potential toxicity and efficacy of proposed pharmaceutical treatment of animals or humans. The imaging system, based on discrete optical components, not only provides a robust and compact tool for real-time measurements, but the modularity of the system also offers flexibility to be applicable to various μCCA structures that may be appropriate to various animal or human models. We investigate the dynamics of doxorubicin, a chemotherapeutic agent, on cultured cells in a μCCA using the integrated cytometric fluorescent imaging system. This study incorporates two uteran cancer cell lines representing a sensitive cell type and a multi-drug resistant (MDR) derivative cell line. The ultimate goal is to test the effect of MDR modulators in combination with doxorubicin to kill cancer cells while not causing undue harm to normal cells.

Paper Details

Date Published: 18 June 2004
PDF: 6 pages
Proc. SPIE 5325, Optical Diagnostics and Sensing IV, (18 June 2004); doi: 10.1117/12.523626
Show Author Affiliations
Donghyun Kim, Cornell Univ. (United States)
Hui Xu, Cornell Univ. (United States)
Sung June Kim, Seoul National Univ. (South Korea)
Michael L. Shuler, Cornell Univ. (United States)

Published in SPIE Proceedings Vol. 5325:
Optical Diagnostics and Sensing IV
Gerard L. Cote; Alexander V. Priezzhev, Editor(s)

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