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Proceedings Paper

Microsphere-based DNA biosensor arrays
Author(s): Linan Song; Jason Epstein; David R. Walt
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Paper Abstract

A microsphere-based DNA biosensor array with high packing density and low detection limits has been previously developed. Polymeric 3.1-μm-diameter microspheres are employed as the detection elements, where each microsphere is functionalized with single-stranded oligonucleotide probe sequences. The biosensor array is fabricated by randomly distributing a stock microsphere suspension, containing various oligonucleotide-functionalized microspheres, on the distal end of a chemically etched imaging fiber bundle. By placing the microspheres into wells at the end of each individual fiber, each optical channel is connected to a single microsphere. The microspheres are encoded with a unique combination of dyes in order to determine a particular microsphere’s location in the randomized array. Specific fluorescence responses are observed after hybridization with fluorescently labeled complementary targets. Enhancement of the signal to noise ratio is possible because of intrinsic redundancy of sensing elements built into the array. This microsphere-based DNA biosensor has several major advantages over existing platforms including higher sensitivity, micron-sized features, and rapid throughput. Microsphere-based DNA arrays have been successfully applied to genomic discrimination of bacteria, gene expression analysis, and the detection of bioagents.

Paper Details

Date Published: 8 March 2004
PDF: 9 pages
Proc. SPIE 5269, Chemical and Biological Point Sensors for Homeland Defense, (8 March 2004); doi: 10.1117/12.516152
Show Author Affiliations
Linan Song, Tufts Univ. (United States)
Jason Epstein, Tufts Univ. (United States)
David R. Walt, Tufts Univ. (United States)

Published in SPIE Proceedings Vol. 5269:
Chemical and Biological Point Sensors for Homeland Defense
Arthur J. Sedlacek III; Richard Colton; Tuan Vo-Dinh, Editor(s)

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