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Proceedings Paper

Cellular analysis based on moving infrared optical gradient fields
Author(s): Philippe J. Marchand; Mark Wang; Catherine Schnabel; Mirianas Chachisvillis; Haichuan Zhang; Rong Yang; Laura McMullin; Norbert Hagen; Osman Kibar; Sadik C. Esener; Gene Tu; Sudipto Sur; Luis Pestana; Kirk Haden; Khai Truong; Wilson Seto; Kris Lykstad; Laura Simons; Michael Paliotti; Ilona Kariv; Jeffrey Hall; William Butler
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Paper Abstract

A novel, non-invasive measurement technique for quantitative cellular analysis is presented that utilizes the forces generated by an optical beam to evaluate the physical properties of live cells in suspension. Analysis is performed by rapidly scanning a focused, near-infrared laser line with a high cross-sectional intensity gradient across a field of cells and monitoring their interaction with the beam. The response of each cell to the laser depends on its size, structure, morphology, composition, and surface membrane properties; therefore, using this technique, cell populations of different type, treatment, or biological state can be compared. To demonstrate the utility of this cell analysis platform we have evaluated the early stages of apoptosis induced in the U937 cancer cell line by the drug camptothecin and compared the results to established references assays. Measurements on our platform show detection of cellular changes earlier than either of the fluorescence-based annexin V or caspase assays. Because no labeling or additional cell processing is required and because accurate assays can be performed with a small number of cells, this measurement technique may find suitable applications in cell research, medical diagnostics, and drug discovery.

Paper Details

Date Published: 10 November 2003
PDF: 9 pages
Proc. SPIE 5181, Wave Optics and Photonic Devices for Optical Information Processing II, (10 November 2003); doi: 10.1117/12.509376
Show Author Affiliations
Philippe J. Marchand, Genoptix, Inc. (United States)
Mark Wang, Genoptix, Inc. (United States)
Catherine Schnabel, Genoptix, Inc. (United States)
Mirianas Chachisvillis, Genoptix, Inc. (United States)
Haichuan Zhang, Genoptix, Inc. (United States)
Rong Yang, Genoptix, Inc. (United States)
Laura McMullin, Genoptix, Inc. (United States)
Norbert Hagen, Genoptix, Inc. (United States)
Osman Kibar, Genoptix, Inc. (United States)
Sadik C. Esener, Genoptix, Inc. (United States)
Gene Tu, Genoptix, Inc. (United States)
Sudipto Sur, Genoptix, Inc. (United States)
Luis Pestana, Genoptix, Inc. (United States)
Kirk Haden, Genoptix, Inc. (United States)
Khai Truong, Genoptix, Inc. (United States)
Wilson Seto, Genoptix, Inc. (United States)
Kris Lykstad, Genoptix, Inc. (United States)
Laura Simons, Genoptix, Inc. (United States)
Michael Paliotti, Genoptix, Inc. (United States)
Ilona Kariv, Genoptix, Inc. (United States)
Jeffrey Hall, Genoptix, Inc. (United States)
William Butler, Genoptix, Inc. (United States)

Published in SPIE Proceedings Vol. 5181:
Wave Optics and Photonic Devices for Optical Information Processing II
Pierre Ambs; Fred R. Beyette Jr., Editor(s)

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