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Proceedings Paper

Novel fluorophores for single-molecule imaging
Author(s): Katherine A. Willets; Oksana Ostroverkhova; Stephan Hess; Meng He; Robert J Twieg; William E. Moerner
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Paper Abstract

A new class of fluorophores has been identified that can be imaged at the single-molecule level and offer additional beneficial properties such as a significant ground state dipole moment, moderate hyperpolarizability, and sensitivity to local rigidity. These molecules contain an amine donor and a dicyanodihydrofuran (DCDHF) acceptor linked by a conjugated unit (benzene, thiophene, alkene, styrene, etc.) and were originally designed to deliver both high polarizability anisotropy and dipole moment as nonlinear optical chromophores for photorefractive applications. Surprisingly, we have found that these molecules are also well-suited for single-molecule fluorescence imaging in polymers and other reasonably rigid environments. We report the bulk (ensemble) and single-molecule photophysical properties measured for six dyes in this new class of single-molecule reporters, with absorption maxima ranging from 486 to 614 nm.

Paper Details

Date Published: 14 November 2003
PDF: 8 pages
Proc. SPIE 5222, Nanocrystals, and Organic and Hybrid Nanomaterials, (14 November 2003); doi: 10.1117/12.506519
Show Author Affiliations
Katherine A. Willets, Stanford Univ. (United States)
Oksana Ostroverkhova, Stanford Univ. (United States)
Stephan Hess, Stanford Univ. (United States)
Meng He, Kent State Univ. (United States)
Robert J Twieg, Kent State Univ. (United States)
William E. Moerner, Stanford Univ. (United States)

Published in SPIE Proceedings Vol. 5222:
Nanocrystals, and Organic and Hybrid Nanomaterials
Alexander N. Cartwright; David L. Andrews; Zeno Gaburro; Charles Y. C. Lee, Editor(s)

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