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Proceedings Paper

Biocompound detection through fluorescence excitation-emission matrix analysis
Author(s): David R. Twede; Lee C. Sanders; Michael L. Wagner
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Paper Abstract

The excitation-emission matrix (EEM) is the luminescence spectral emission intensity of fluorescent compounds as a function of the excitation wavelength. EEMs offer the promise of an additional degree of information for enhanced compound detection and identification. Veridian has collected pure-component EEMs of amino acids (Trp, Phe, Tyr), Bacillus globigii (bg), Bacillus thuringiensis (bt,), and selected backgrounds. Also collected were EEMs of mixtures of amino acids and of bg in solution with a few backgrounds. The EEMs of pure components and mixtures were analyzed for phenomenology and for potential methods of unmixing and identifying the constituents of EEMs having mixed components of a similar nature.

Paper Details

Date Published: 7 January 2004
PDF: 7 pages
Proc. SPIE 5159, Imaging Spectrometry IX, (7 January 2004); doi: 10.1117/12.506169
Show Author Affiliations
David R. Twede, Veridian Systems Div. (United States)
Univ. of Michigan (United States)
Lee C. Sanders, Veridian Systems Div. (United States)
Michael L. Wagner, Veridian Systems Div. (United States)

Published in SPIE Proceedings Vol. 5159:
Imaging Spectrometry IX
Sylvia S. Shen; Paul E. Lewis, Editor(s)

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