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Proceedings Paper

Analysis of neuropeptides using capillary zone electrophoresis with multichannel fluorescence detection
Author(s): Jonathan V. Sweedler; Jason B. Shear; Harvey A. Fishman; Richard N. Zare; Richard H. Scheller
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Paper Abstract

Capillary zone electrophoresis is fast becoming one of the most sensitive separation schemes for sampling complex microenvironments. A unique detection scheme is developed in which a charge-coupled device (CCD) detects laser induced fluorescence from an axially illuminated electrophoresis capillary. The fluorescence from an analyte band is measured over a several centimeter section of the capillary, greatly increasing the observation time of the fluorescently tagged band. The sensitivity of the system is in the 1-8 X 10-20 mol range for derivatized amino acids and peptides. Subattomole quantities of bag cell neuropeptides collected from the giant marine mollusk Aplysia californica can be measured.

Paper Details

Date Published: 1 December 1991
PDF: 10 pages
Proc. SPIE 1439, International Conference on Scientific Optical Imaging, (1 December 1991); doi: 10.1117/12.50463
Show Author Affiliations
Jonathan V. Sweedler, Stanford Univ. (United States)
Jason B. Shear, Stanford Univ. (United States)
Harvey A. Fishman, Stanford Univ. (United States)
Richard N. Zare, Stanford Univ. (United States)
Richard H. Scheller, Stanford Univ. (United States)

Published in SPIE Proceedings Vol. 1439:
International Conference on Scientific Optical Imaging
M. Bonner Denton, Editor(s)

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