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Proceedings Paper

Digital imaging microscopy: the marriage of spectroscopy and the solid state CCD camera
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Paper Abstract

Biological samples have been imaged using microscopes equipped with slow-scan CCD cameras. Examples are presented of studies based on the detection of light emission signals in the form of fluorescence and phosphorescence. They include applications in the field of cell biology: (a) replication and topology of mammalian cell nuclei; (b) cytogenetic analysis of human metaphase chromosomes; and (c) time-resolved measurements of DNA-binding dyes in cells and on isolated chromosomes, as well as of mammalian cell surface antigens, using the phosphorescence of acridine orange and fluorescence resonance energy transfer of labeled lectins, respectively.

Paper Details

Date Published: 1 December 1991
PDF: 12 pages
Proc. SPIE 1439, International Conference on Scientific Optical Imaging, (1 December 1991); doi: 10.1117/12.50457
Show Author Affiliations
Thomas M. Jovin, Max Planck Institute for Biophysical Chemistry (Germany)
Donna J. Arndt-Jovin, Max Planck Institute for Biophysical Chemistry (Germany)

Published in SPIE Proceedings Vol. 1439:
International Conference on Scientific Optical Imaging
M. Bonner Denton, Editor(s)

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